Effects of Nutrition on Academic Performance Essay

Introduction Some students are unaware as to what they should expect going into post-secondary education. The student may be right out of high school, were their teacher ‘held their hand’ the entire time, been out of school for a long period of time, or may even have dropped out of a previous program and trying something new. Post-secondary education isn’t like high school or working a full-time job, it is a lot of dedication and hard work. Pursuing in post-secondary education will improve your knowledge base in a particular area you have chosen to improve on and prepare you for success with your future career goal. Students are entering into college and university to further their education, with the expectations to graduate and†¦show more content†¦Many elementary schools across Canada have a breakfast program provided for children in need and are available right before the school day starts. The program helps educate students, at an early age, on the importanc e of eating nutritious food to start their day. Problem Statement Knowing the effects of nutrition on your brain growth and consuming what is necessary for brain function and growth will better prepare you for successful academic completion. Studies have shown that â€Å"students who had better nutritional quality and habits were ‘significantly less likely to fail the literacy assessment[s]’ (Florence et al. 2008)† (Vautier 2012). As the day progresses, must students believe caffeinated beverages will give them the energy they require to get through their day. However, consuming caffeinated beverages and food may temporary assist in an energy boost, but this will to ‘crashes’ later on in the day. More consumption of these caffeinated beverages and food will result in repeating what your body just went through. This cycle of high boost of energy will follow by a drastic energy loss. It is suggested by nutritionist to eat proper food instead, such as fruits and vegetables, to give your body the energy that it re quires instead of caffeine. Another issue on student academic performance is entering into post-secondary education without a goal, which may lead toShow MoreRelatedEffect of Nutrition on Academic Performance895 Words   |  4 PagesEffect of Nutrition on Academic Performance RGS6035.E2 - Chapter 1 Kurt Cornett Amberton University Effect of Nutrition on Academic Performance Every year millions of tax dollars are spent on school nutrition programs all over the United States. Legislators across the nation lobby for coordinated school health programs and place increasing emphasis on student nutrition. Television commercials remind kids to eat a balanced diet and food products aimedRead MoreThe Findings From My Current Research Indicate That It1697 Words   |  7 PagesThe findings from my current research indicate that it is true that poor nutrition negatively affects the academic performance of the students. Also, the study shows that when the students are given meals with good nutrient content, it is significant because it supports their growth and development as well as improve their cognitive development (Langford, et. al, 2014). The study showed that majority of the students who performed poorly in their tests are the one that is fed in meals that lack nutrientsRead MoreBharadwaj, Là ¸ken, Neilson (2013), Have Revealed That911 Words   |  4 Pag esof research has been put on the relationship between the brain function and nutrition. The brain needs a proper diet which improves its performance and function (Bharadwaj, Là ¸ken, Neilson, 2013). More than 60 percent of the human brain is made up of fat which plays a role of the messenger that control aspects such as mood. For instance, the Omega-3 fatty acids have been found to be significant for the optimal performance of the brain, and when a person lacks this kind of fatty acids, he or she mayRead More Psychology Studies Essay1522 Words   |  7 PagesTwo-Year School Breakfast Program for Preschool-Aged Children on Their Nutrient Intake and Pre-Academic Performance. Child Study Journal, 29, 113-131. This study contains information dealing with the relationship between nutrition and academic performance. The A variable consists of eating a well-balanced breakfast with a School Breakfast Program (SBP), while the B variable consists of pre-academic performance. The procedure these researchers used to study a nutritional breakfast was to provide preschoolersRead MoreEssay on Breakfast as a Scholastic Tool1448 Words   |  6 Pageshowever, may bear more significant than was ever thought before. Researchers now believe that breakfast indeed is the most important meal of the day, resulting in academic and psychosocial improvements that last a lifetime. While many may have trouble believing that a piece of toast in the morning can produce increased academic performance as well as a healthy mind, the fact is it has been proven to be true. It is for this reason that the federal government should establish a universal free breakfastRead MoreDo Children That Eat Breakfast?1701 Words   |  7 Pagesfinished their homework†.(Better Grade Through Breakfast Vibrant Life Mar.2001:8 Health Reference Center Academic. Web.1.Nov 2015). Research also indicates that children who skip on an average on breakfast at least a couple of hours a week develop behavioral problems is what studies are indicating and that is due to lack of concentration and behavior problems that develop. Nutrition also plays a role in how we function and develop when it comes to eating breakfast or not. Research indicatesRead MoreThe Effect Of Poor Nutrition On The Student s Academic Achievements1606 Words   |  7 PagesIntroduction The purpose of this research paper is to examine the impact of poor nutrition on the Student’s academic achievements. Therefore, this chapter presents and describes the methodology that is used in completing this research paper. The methodology will include the systematic as well as theoretical analysis of methods that will be applied in collecting data, and this is significant in proving whether the hypotheses have been confirmed or not. Inappropriately describe the methodology usedRead MoreLife Chances Of Poor Children Essay1423 Words   |  6 Pages and at least some of these problems might be addressed without substantial increase in resources.† Children in poverty need special support in order to achieve academic success. These flaws will only hold these children back from succeeding. Research Question: Are government assistance programs helping improve the academic performance of children in poverty? Methods Sample The population being studied in this research is families currently living in poverty with middle school age childrenRead MoreNutrition : A Healthy Health, Growth And Development1065 Words   |  5 PagesOne of our goals as parents is ensure we provide adequate care and nutrition for our children. Most parents know that proper nutrition of their baby is imperative, since proper nutrition promotes a healthy life. For that reason, parents may find themselves having to make some tough decisions when it comes to feeding their children. Proper nutrition of children is the key to determine good health, growth and development. In this article, I will provide parents with information to help them make theRead MoreChildren ´s Diet and Nutrition, An Anotated Bibliography Essay1156 Words   |  5 PagesBauer, K. W., Berge, J. M., Larson, N., Neumark-Sztainer, D. (2011). Are Parents of Young Children Practicing Healthy Nutrition and Physical Activity Behaviors? Pediatrics, 127(5), 881-887. doi: 10.1542/peds.1010-3218. The authors of this article present an investigation on the changes in health behaviors during parenthood. The study examines health behaviors, such as nutrition and physical activity, of both parents and young adults without children. Based on the findings, the authors concluded

One Tragic Defeat Free Essays

The poem, Sir Gawain and the Green Knight, illustrates the perfection of a knight throughout his life. Sir Gawain the perfect knight goes on a Christmas game quest provided by the Green Knight which tempts his purity and eventually ruins the ideal knight he used to be. In the criticism, â€Å"A Psychological Interpretation of Sir Gawain and the Green Knight†, the critic Stephen Manning argues that the poem centers on Gawain’s feeling of guilt. We will write a custom essay sample on One Tragic Defeat or any similar topic only for you Order Now On the other hand, P. J. C. Field a critic who wrote, â€Å" A Rereading of Sir Gawain and the Green Knight†, argues that Gawain’s sin in accepting the lady’s girdle is minimal. The remainder of the criticism portrays the comparison between the two critics mentioned. The feeling of guilt occurred once during Sir Gawain’s life; this one time happened to be the day a faultless knight receives his first sin. Sir Gawain holds the situation seriously, and it affects the rest of his life. For example, the green belt Gawain wears, â€Å"symbolizes both [his] shame and his self-knowledge† (Manning 158). Manning explains the color green as a symbol of rebirth, therefore the green belt Sir Gawain carries around resembles the revival of on his short comings. Gawain discovers he is not perfect and learns from his mistakes, thus he becomes a finer, more superior knight which he wishes to become. For instance, Gawain illustrates himself as evil thus informs his peers, â€Å"for evil to exist, it must exist in the good† (159). Manning describes Gawain as a perfect person, one without sin, as if he were a god. Gawain’s peers strive to become like him, so his sin exhibits the impurity and imperfection of human kind. Gawain reveals to his peers that everyone makes mistakes, and should not dread or guilt over them. Guilt demonstrates the psychological feelings of Sir Gawain in the poem. Accepting the girdle for the Lord’s lady is temptation, therefore a mortal sin, but for the predestination of oneself, keeping the girdle was a hard decision to make. Venial sin refers to sin that accidentally happens, whereas mortal sin conveys a sin as one that was supposed to happen. If one commits mortal sin, she or he must visit a priest for penance; but if one commits a venial sin, she or he must ask for forgiveness through prayer. Altogether when anyone asks for forgiveness he or she is forgiven and remains pure. Thus, Gawain only commits a venial sin but, â€Å"venial sin is evil: absolutely, for a man who wants perfection; for a man who is near perfection; and for a man who is possibly†¦ habitually free even from venial sin† (Field 260). Manning argues that to Gawain, a venial sin is evil because he has never committed a sin in his flawless life as a knight. Manning also acknowledges that Gawain takes his first sin solemnly and holds it against himself. He eventually notices that all the pain and suffering he has been through had a reason. Consequently, Gawain returns with a green belt to, â€Å"the court to which [he] returns must be taken as giving the judgement of humanity† (261). Manning implies that through Gawain, everyone in the court shall learn from his mistake and should be prepared and knowledgeable in the future. Through the suffering of a noble and perfect knight, everyone benefits from their courteous peer. Entirely, the acceptance of the lady’s girdle was a venial sin or minimal sin because he did it for the sake of keeping his life. The acceptance of the lady’s girdle led to the guilt of Sir Gawain and the judgement of others. â€Å"But accepting the girdle is not a sin in the theological sense† (Manning 157). Manning is insinuating that the girdle is not a mortal sin but instead a chivalric sin. Therefore Gawain should not be humiliated through guilt as it is not a theological sin, which makes him a perfect man religiously but not through the state of chivalry. How to cite One Tragic Defeat, Essay examples

Academic Research Writing

Question: Discuss about theAcademic Research Writing. Answer: Introduction Academic writing refers to the act of publishing a conducted research that is set in several subsets of forms and genres. This article sets out to provide a full spectrum of the critical components of academic writing and also gives examples of academic writings. The paper will set out to present an overview of the critical approaches applicable when one does a piece of about a subject. Much as writing is always seen to be influenced by the social context in which the literature is produced, it is highly influenced by the thought processes that writers go through before doing the actual writing or in the course of their writing. Research conducted on the role of cognition in writing has it that the writing processes have a great role in shaping how writers think about what they write. The research further shows that the transformation of integrated word forms part of the integral component of the thoughts therein and as such, forms part of the fundamental processes of the most skilled writers. According to Bergmann, (2008), one cannot single out any academic literacy based on the far-fetched variations that exist between the genres. When carrying out academic writing, one does so in a dispassionate and impersonal tone, usually targeted for an informed and critical audience that is more often than not, informed on a strictly established knowledge, and meant to challenge or enforce some concepts or arguments. The writing usually revolves around and within the academic world. Nonetheless, an academic writer may also find his or her audience outside this establishment through written speeches, pamphlets, and journalism among other media. In English as a subject, some elements of ambiguity often emanate from the multifaceted nature of the literacy texts. According to Preece, (1998), uncertainty, ambiguity, and indeterminacy are all integral components of literacy expression and often form part of the fictional texts that portray their own meaning. Belcher et. al., (1985) on his part maintains that the ambiguity is an integral component of the literature that depicts the quality of the piece of work and helps in distinguishing the reading text from the interpretation. He closes by saying that the above assertion has a bearing on the failures as captured by the reading. When students are set to come up with a comprehensively researched academic work, they must always take into account the fact that evaluative judgment of their course and experience is essential. This is because multiple issues including interpretation may be varied from one individual to another and as such, may be more plausible than others. Notably, coming up with a well-documented and compelling argument can be very useful in ensuring that one develops an idea by use of referenced information. By all standards, an academic writing must always have an objective stance that clearly defines the significance of the topic of discussion. This is usually organized with some adequacy so as to enable other scholars to attempt replicating the findings. It should be noted that substantial scholarly papers are often not overly seen as general but instead makes use of the formal academic rhetoric when need be. How can one Achieve a good Research Paper? For one to achieve this, it is important to have in place a well worked out plan that involves stages. The actual writing process is often hectic, messy and recursive if one is not very careful. The given outline will help through the writing process and ensure that the outcome is palatable in all respects. The first step includes discovering, narrowing down and focusing on the researchable topic. The next stage will be finding and selecting the various resource materials. Grouping and sequencing of the information to be researched about is then documented. What follows is the outline of the subject matter together with ones prospect. Having these in place, it becomes imperative to have an introduction of the paper, a thesis statement which captures the key ideas of the write-up and then the body. This is where the details concerning the research work are obtained including previous studies. When this is done, the conclusion of the paper comes in where the writer gives his or her reflection of the paper giving out the final thoughts of the written research. Having all in one place, the author then goes through the written draft proofreading all the work through to ascertain that it is error-free (Wallwork, 2016). Proofreading is critical in the sense that it helps in improving the general structure of the written work, checking the overall organization of the paper. It also helps in getting rid of paragraph level concerns. Lastly, it arrests any sentence level concerns and grammatical mistakes. Conclusion The efforts to improve the writing prowess among students in so far the academic writing is concerned should be able to instill in them greater skills and attention to the role subjective arguments play in their interpretive instances as they come up with a well-researched work. The genre-based methods to teaching writing tend to emphasize the importance of helping students through the process of understanding various styles and being able to distinguish them from one another. Notes Bergmann, Linda. Academic Research: Fields of Study and Inquiry. New York: Longman, 2008. Belcher, Diane Dewhurst, and George Braine. Academic Writing in a Second Language: Essays Haisler, Peter. How to Write a Good Research Paper. Frederiksberg: Samfundslitteratur, 2011.on Research and Pedagogy. Norwood, NJ: Ablex Pub. Corp, 1995. Monippally, Mathukutty M., and Badrinarayan Shankar Pawar. Academic Writing: A Guide for Management Students and Researchers. New Delhi: SAGE India, 2010. https://public.eblib.com/choice/publicfullrecord.aspx?p=543959. Preece, Roy A. Starting Research: An Introduction to Academic Research and Dissertation Writing. London [u.a.]: Pinter, 1998. Wallwork, Adrian. English for academic research, writing exercises. Berlin: Springer, 2012. Wallwork, Adrian. English for Writing Research Papers. 2016.

Taylor Poe Essay Example For Students

Taylor Poe Essay The artist formally known as the Perfectionist had a very good year going for him. He won the 2001 Brawl for it All to become number one contender for Archadias EWF Title, but the merger of EWF and USW canceled any chances he had of winning that. Was thought to be the odds on favorite to win the Owen Hart Memorial Tournament, but no showed, thus throwing his chance at the Title out the window. Eventually did debut in the UWA and gained a title shot at Champion Matt Mitchell, but was abducted by the United States Navy and was shipped off to Afghanistan before he got his shot. He now resides there and tries to and kill Osama Bin Laden. We here at the United Wrestling Alliance wish him the best of luck. We will write a custom essay on Taylor Poe specifically for you for only $16.38 $13.9/page Order now The artist formally known as the Perfectionist had a very good year going for him. He won the 2001 Brawl for it All to become number one contender for Archadias EWF Title, but the merger of EWF and USW canceled any chances he had of winning that. Was thought to be the odds on favorite to win the Owen Hart Memorial Tournament, but no showed, thus throwing his chance at the Title out the window. Eventually did debut in the UWA and gained a title shot at Champion Matt Mitchell, but was abducted by the United States Navy and was shipped off to Afghanistan before he got his shot. He now resides there and tries to and kill Osama Bin Laden. We here at the United Wrestling Alliance wish him the best of luck. The artist formally known as the Perfectionist had a very good year going for him. He won the 2001 Brawl for it All to become number one contender for Archadias EWF Title, but the merger of EWF and USW canceled any chances he had of winning that. Was thought to be the odds on favorite to win the Owen Hart Memorial Tournament, but no showed, thus throwing his chance at the Title out the window. Eventually did debut in the UWA and gained a title shot at Champion Matt Mitchell, but was abducted by the United States Navy and was shipped off to Afghanistan before he got his shot. He now resides there and tries to and kill Osama Bin Laden. We here at the United Wrestling Alliance wish him the best of luck.

The Surprising History of Black People in Germany

The Surprising History of Black People in Germany The German census does not poll residents on race, following World War II, so there is no definitive number of the population of black people in Germany. One report  by  the European Commission against Racism and Intolerance  estimates there are 200,000 to 300,000 black people living in Germany, although other sources guess that number is higher, upwards of 800,000.   Regardless of the specific numbers, which dont exist, black people are a minority in Germany, but they still are present and have played an important role in the countrys  history.  In Germany, black people are typically referred to as Afro-Germans (Afrodeutsche) or black Germans (Schwarze  Deutsche).   Early History Some historians claim that the first, sizable influx of Africans came to Germany from Germanys African colonies in the 19th century. Some black people living in Germany today can claim ancestry dating back five generations to that time. Yet Prussias colonial pursuits in Africa were quite limited and brief (from 1890 to 1918), and far more modest than the British,  Dutch and French powers. Prussias South West African colony was the site of the first mass genocide committed by Germans in the 20th century. In 1904, German colonial troops countered a revolt with the massacre of three-quarters of the Herero population in what is now Namibia. It took Germany a full century to issue a formal apology to the Herero for that atrocity, which was provoked by a German extermination order (Vernichtungsbefehl). Germany still refuses to pay any compensation to the Herero survivors, although it does provide foreign aid to Namibia.   Black Germans Prior to World War II After World War I, more blacks, mostly French Senegalese soldiers or their offspring, ended up in the Rhineland region and other parts of Germany. Estimates vary, but by the 1920s, there were about 10,000 to 25,000 black people  in Germany, most of them in Berlin or other metropolitan areas. Until the Nazis came to power, black musicians and other entertainers were a popular element of the nightlife scene in Berlin and other large cities. Jazz, later denigrated as Negermusik (Negro music) by the Nazis, was made popular in Germany and Europe by black musicians, many from the U.S., who found life in Europe more liberating than that back home. Josephine Baker in France is one prominent example. Both the American writer and civil rights activist W.E.B. du Bois and the suffragist Mary Church Terrell studied at the university in Berlin. They later wrote that they experienced far less discrimination in Germany than they had in the U.S. The Nazis and theBlack Holocaust When Adolf Hitler came to power in 1932, the racist policies of the Nazis impacted other groups besides the Jews. The Nazis racial purity laws also targeted gypsies (Roma), homosexuals, people with mental disabilities  and black people. Precisely how many black Germans died in Nazi  concentration camps  is not known, but estimates put the figure at between 25,000 and 50,000. The relatively low numbers of black people in Germany, their wide dispersal across the country and the Nazis focus on the Jews were some factors that made it possible for many black Germans to survive the war.   African Americans in Germany The next influx of black people to Germany came in the wake of World War II when many African-American GIs were stationed in Germany.   In Colin Powells  autobiography My American Journey, he  wrote  of  his tour of duty in West Germany in 1958 that for ... black GIs, especially those out of the South, Germany was a breath of freedom -   they could go where they wanted, eat where they wanted and date whom they wanted, just like other people. The dollar was strong, the beer good, and the German people friendly. But not all Germans were as tolerant as in Powells experience. In many  cases, there was resentment of the black GIs having relationships with white German women. The children of German women and black GIs in Germany were called occupation children† (Besatzungskinder) - or worse.  Mischlingskind  (half-breed/mongrel child) was one of the least offensive terms used for half-black children in the 1950s and 60s.   More About the Term 'Afrodeutsche' German-born blacks are sometimes called Afrodeutsche (Afro-Germans)  but the term is still not widely used by the general public. This category includes people of African heritage born in Germany. In some cases, only one parent is black But just being born in Germany does not make you a German citizen. (Unlike many other countries, German citizenship is based on the citizenship of your parents and is passed on by blood.) This means that black people born in Germany, who grew up there and speak fluent German, are not German citizens unless they have at least one German parent. However, in 2000, a new German naturalization law made it possible for black people and other foreigners to apply for citizenship after living in Germany for three to eight years. In the 1986 book, Farbe Bekennen  -   Afrodeutsche Frauen auf den Spuren Ihrer Geschichte, authors May Ayim  and  Katharina Oguntoye  opened up a debate about being black in Germany. Although the book dealt primarily with black women in German society, it introduced the term Afro-German into the German language  (borrowed from Afro-American or African American) and also sparked the founding of a support group for blacks in Germany, the ISD (Initiative Schwarzer Deutscher).

How Much House Can You Afford Learn to Calculate a Mortgage

How Much House Can You Afford Learn to Calculate a Mortgage SAT / ACT Prep Online Guides and Tips If you’re looking to buy a new home, then you’re likely asking yourself, how much house can I afford? How much mortgage can I afford, and what’s a reasonable monthly payment to take on? You don’t want to get in over your head, but you also don’t want to live in a shoebox in the middle of nowhere. Where does the balance lie? This guide will help you answer the question, "How much house can I afford?" based both on the numbers and your overall happiness. To begin, let's go over some general guidelines to consider when buying a home. Buying a Home: General Guidelines There’s a common misconception around buying a house, and it’s that you should buy the biggest and best house you can afford. However, the house you can buy and the house you should buy are not necessarily the same thing. Some banks will lead you right up to the precipice of spending when they offer you a mortgage. They’ll give you a loan that you can just afford to pay, but any big life changes, like getting fired or ill, could push you over the edge into bankruptcy. Not only is taking on the biggest mortgage you can financially risky, but it also may not do much for your overall life satisfaction. When it comes to house size and location, what we think we need and what actually fulfills us don't always match up. Figuring out the answer to "how much house can I afford" isn’t just about facts and figures. It also requires that you get introspective and strive to make the best financial and emotional decision based on your resources and needs. Before exploring issues of personal needs in greater depth, let’s take a look at the hard numbers. What percentage of your income should go toward housing payments? Deciding on a house is a balancing act between your financial and emotional needs. Key Rule of Thumb: Spend No More Than 30% of Your Income To figure out, how much home can I afford, the conventional wisdom tells us to spend up to 30% of our gross income on housing a year, or a little less than one-third. Gross income is what we make before taxes, not our actual take-home pay. We use gross income because the interest you pay on a mortgage is tax deductible. Regardless of whether you’re making $20k a year or $2 million a year, you should stick to this rule of thumb. You don’t want to overcommit to home ownership payments in case of an emergency, like losing your job or a costly injury. So, how can you calculate the house price that would correspond to 30% of your income? It's not just three times your income, because you have to consider total housing costs, which include the mortgage principal, mortgage interest, and general maintenance costs. Consider the example below. Remember the golden rule of real estate: spend no more than 30% of your annual income on housing costs. Estimating House Price: An Example You don't necessarily need a mortgage calculator to answer the question "how much mortgage can I afford?" When figuring out how expensive of a house you can buy, you can estimate that the lump sum house price can go up to roughly five times your total income. Why? The average person gets a 30-year mortgage and pays off 6% of the house price each year. This percentage may vary depending on your exact agreement (typically somewhere between 4% and 8% of the house price), but it's an average that applies to lots of people. This payment refers to total housing costs, which include both the mortgage and other costs. If 6% of the total housing costs equals about 30% of your income (using the 30% rule of thumb discussed above), then the total house price equates to about five times your income. We can represent this using the variable "h" for house price and "i" for income. Here’s how the math works out: 0.06h = 0.3i Divide both sides by 0.06 h = 5i Let’s say you make about $40,000 per year. Using this guideline, you can estimate that you can afford a house up to $200,000. While you can spend up to approximately five times your income on a house, you could also choose to purchase a less expensive home (then you’ll have more disposable income for golf or spontaneous trips to Paris!). Once you figure out the mortgage, or loan, you need, you’ll apply through a bank. Below are three steps you can take to qualify for your mortgage and reduce your monthly payments. Besides crunching the numbers, how else can you prepare to buy a house? Qualifying for a Mortgage: Steps You Should Take Unless you’re paying for the house with a suitcase full of cash and gold bars, you’ll need to apply for a mortgage. Most mortgages have fixed interest rates somewhere between 2.6% and 3.7%, and people commonly choose a repayment plan that spans 30 years. Once you reach this step, your next questions might be, how much mortgage can I afford and how much can I borrow? Banks look at three main factors when determining your mortgage and repayment plan. The first is how much you pay up front as a down payment. The second is your outstanding debts, and the third is your credit score. These are the three steps you can take to put yourself in the best financial position for getting a mortgage and buying a house. Step #1: Save for a Large Down Payment The more money you can pay on a house up front, the less you’ll have to pay per month. With reduced monthly payments and perhaps a shorter term, you also won’t have to pay as much on interest in the long run. Aim to pay at least 10% of the total house price as a down payment. Ideally, you can pay 20%. On a $300,000 house, for example, a 20% down payment would be $60,000. Some banks charge an extra monthly payment toward Private Mortgage Insurance (PMI) if you have less than a 20% down payment. Banks want to avoid risk, and a low down payment is one sign of risk. The bigger your down payment, the better contract you’ll get with the lender. The bigger down payment you can put down up front, the better! Step #2: Pay Down Your Debts Another way that banks assess risk is by looking at your outstanding debts. These debts might include student loans, car loans, or other personal loans. The less debt you have, the better deal you’ll get. If you have a huge monthly payment toward student loans, for instance, then you may not appear to be in a good position to put money toward a mortgage. You should also strive to show a history of consistent, on-time payments. If you’ve let your loans go into default, then you may not get approved for a mortgage. If you’re planning on buying a house, then it’s important that you handle your debts responsibly and work toward reducing them. Step #3: Build Up Your Credit Score Finally, your credit score is an important factor when you apply for any kind of loan. Lenders look at your financial behavior in the past as an indication of your financial behavior in the future. The strongest credit scores fall into the upper 700s or 800s. Now that you have a sense of the numbers - paying up to 30% of your income on housing - what else should you think about as you venture down the path toward home ownership? Buying a House: Beyond the Numbers A lot of guides for first time homeowners will urge you to go out and find a house that costs about five times your annual income (or the combined incomes of you and your spouse). While this mortgage calculator estimate may work well for you, assuming no unexpected interruptions in your income, it’s not necessarily the end all and be all in real estate advice. This mindset urges people to step right up to the limits of their financial profile to get the biggest house in the best location that they can. It prompts you to answer, how much house can I afford, with the biggest possible number. Banks and realtors, of course, are motivated to keep people in this maximizing mindset. But is this way of thinking necessarily the best way to approach buying a house? How much mortgage can I get and how much mortgage should I get are two different questions. If you think you’ll be happiest with the biggest or most centrally located house you can get, you may be overestimating the importance of house size and location. Let’s consider both of these factors, amount of space and location, individually. If your realtor shows you places like this, you might remind her that you probably don't need quite this much space for a three-person family. How Big a House Do You Want? People often want to maximize the amount of space they can afford, but they may not be happier with more space in the long run. Humans have a knack for adjusting, which can have many benefits, but also means that we won’t stay satisfied with material improvements in our surroundings for long. According to UC Riverside psychology professor, Sonja Lyubomirsky, â€Å"Someone who feels elated after upgrading to a big house is likely to soon start yearning for more - an extra bedroom, a pool, whatever it may be. But ultimately, whether we drive a battered truck or a Lexus to work; whether we have hypertension or asthma, our ability to be happy and get happier doesn’t vary much.† As long as we have a certain amount of comfort and our needs our being met, we won’t get much happier from a bigger house in the long run. People tend to acclimate to the space that they have, and many report higher levels of happiness after minimizing their lifestyles. All too often, extra space just becomes storage for extra stuff. You don't need to donate all your possessions and join the tiny house movement, but you should take time to think back on your various living spaces. Were you substantially happier in larger spaces? What was the smallest space you’ve lived in and felt satisfied? Before falling prey to the McMansion mindset, think about how much space would actually make you happy and how much would be unnecessary excess. Where Should Your House Be Located? While the size of your house may not be as important as you think, its location can have a large effect on your happiness levels for one main reason: your commute to work. Several studies have shown that commute time has a big impact on personal well-being. A study conducted at the University of Waterloo concluded that long commutes link with low overall satisfaction in life, while a 20 study from Sweden connected long commutes with low energy, high stress, and more frequent absences from work due to illness. While you may not be able to afford a location with the best commute, you should avoid buying a house far away from your place of work. Finding the right house becomes a balance, then, among your finances, the size of the house, and the area where it’s located. We all want to live in Danny Tanner’s townhouse from Full House, but with the average cost of a 4-bedroom house in San Francisco going for over $1.7 million, most of us have to let this dream go. How Much House Can I Afford? Final Thoughts There are lots of steps that go into buying a house, and you have to take a close look at your finances and personal needs. Generally speaking, you should spend no more than 30% of your income on housing payments (mortgage plus the other costs of home ownership). You can use this rule of thumb to look at houses that cost five times your annual income or less. While banks might be all too eager to grant you a large mortgage - assuming you can put down a large down payment, are paying down your debts, and have a strong credit score - you shouldn’t necessarily take on the biggest mortgage you can. Taking on any kind of debt is a risk, and you should be cautious about over-committing to a 30-year mortgage if you’re concerned about things in your life, like job security. You should also consider how much of your monthly income you need for spending in other categories, like a retirement savings account or a January vacation to the Caribbean. Think about what will make you happiest, whether it’s a larger space, a short commute, a location close to restaurants and cafes, or a small monthly payment that frees up your income for leisure activities. When purchasing a home, you need to think long-term about both your finances and your overall happiness. Once you’ve figured out what you’re looking for, you can make steps to put yourself in the best position for getting a mortgage and buying your perfect house.

Organizational Change Theory Research Paper Example | Topics and Well Written Essays - 1250 words

Organizational Change Theory - Research Paper Example In this paper, we will explore some key issues related to the theory of organizational change. Importance of Organizational Change Theory By understanding organizational change theory, managers can explore various external and internal forces of change. Two main external factors of change include competitive environment and technological development. Competitive environment is such a factor that forces an organization to ensure development of high quality products to increase customer base, as well as to improve customer satisfaction. Technological development, on the other hand, refers to the use of latest technology to remain competitive in the market. If we talk about the importance of organizational change theory with respect to internal forces of change, we can say that this theory helps managers understand changes in the working atmosphere, changes in employment rules and regulations, and required changes in employee retention strategies. As Rasing (2010) states, â€Å"the key to organizational change and development lies in the understanding of people's requirements and work towards it† (p. 1). ... 49). From employers’ perspective, the readiness to adapt to ongoing internal and external changes is the biggest thing that organizational change theory demands from employers. Moreover, managers also need to show willingness for organizational changes, as well as for developing strategies for implementing those changes. A higher level of organizational readiness for changes results in more effective implementation of required changes. The reason is that when the level of readiness is high, employers take quick actions to initiate the process of change, as well as display a highly cooperative behavior with all members of the organization. Organizational members need to be committed to towards implementing essential changes to organizational policies and functions in order to maximize the levels of productivity and efficiency. Employers also need to assess the key determinants of change implementation, which include situational factors, task demands, and availability of require d resources. These three determinants play a vital role in determining whether an organization is ready to implement required changes or not. Based on assessment, employers develop suitable strategies to put required changes into action. Demands of Organizational Change: From Employees’ Perspective If we talk about the requirements of organizational change from employees’ perspective, we can say that willingness to work according to modified policies and eagerness to get trained accordingly are the biggest requirements. Employees always play a critical role in bringing positive organizational changes. As Avey, Wernsing, and Luthans (2008) states, â€Å"one of the

Health Insurance Portability and Accountability Act's Ethics Essay

Health Insurance Portability and Accountability Act's Ethics - Essay Example A standard electronic device should be used to store all medical information of a person. The deadline for embracing the act was September 23, 2013. The health policy incorporates some ethical issues. These ethical issues include respect for the patient’s health information, confidentiality and honesty. HIPAA law has bought has established responsibilities for health care institutions. The HIPAA law has enhanced the confidentiality of the patients’ medical information. Confidentiality is the state of having something in a strict privacy or secrecy way. This helps to protect patient rights since only the required medical personnel’s can access the information. This is a component of providing quality medical health care. In a recent case, a patient suffering from paranoid schizophrenia got admitted to a mental hospital. The nurses at the hospital wanted to disclose the health information to the family. However, the patients’ physician refused since the patient had not allowed them to do so. The patients’ health rapidly improved, and within a week, he was ready for discharge. The patients’ health condition requires maximum respect. Respect is the condition of esteem. In the case of ongoing treatment, obtaining payments and for healthcare operations, one can share protected health information. Except for insurance reasons in order to receive payments written authorization should be presented any time one requires medical records. Under this condition HIPAA only allows access of minimum necessary information to perform job duties. This enhances respect for patients’ health information. In another case, a staff member in a medical practice disclosed HIV status of the patient. This happened mistakenly after the doctor’s office faxed medical records to the patients’ place of employment rather the new healthcare provider. He thereby disclosed PHI to several other individuals.

7 Days of Prayers for the Souls in Purgatory Essay Example for Free

7 Days of Prayers for the Souls in Purgatory Essay PREPARATORY PRAYER My Jesus, by the sorrows You suffered in Your agony in the Garden, in Your scourging and crowning with thorns, in Your journey to Calvary, in Your crucifixion and death, have mercy on the souls in purgatory, and especially on those that are most forsaken; deliver them from the torments they endure; call them and admit them to Your most sweet embrace in paradise, where You live with the Father and the Holy Spirit, one God, for ever and ever. Amen See more: Homeless satire essay Prayers from The Raccolta (Here say the prayer for the day) 0ur Father Our Father in Heaven, Your Kingdom come; Your will be done on earth, as it is in Heaven. Give us this day our daily bread; and forgive us our sins, as we forgive those who sin against us; and lead us not into temptation, but deliver us from evil. Amen. Hail Mary Hail Mary, full of grace, the Lord is with you; blessed are you among women; and blessed is the fruit of your womb, Jesus. Holy Mary, Mother of God, pray for us, sinners, now and at the hour of our death. Amen. Eternal Rest Eternal rest, grant unto them, O Lord; and let perpetual light shine upon them. May they rest in peace. Amen. Concluding Prayer Merciful Father, hear our prayers and console us. As we renew our faith in Your Son, whom You raised from the dead, strengthen our hope that all our departed brothers and sisters will share in His resurrection, who lives and reigns with You and the Holy Spirit, one God, for ever and ever. Amen. SUNDAY O Lord God omnipotent, I beseech You by the Precious Blood, which Your divine Son Jesus shed in the Garden, deliver the souls in purgatory, and especially that one which is the most forsaken of all, and bring it into Your glory, where it may praise and bless You forever. Amen. Our Father, Hail Mary, Eternal rest, etc. MONDAY O Lord God omnipotent, I beseech You by the Precious Blood, which Your divine Son Jesus shed in His cruel scourging, deliver the souls in purgatory, and among them all, especially that soul which is nearest to its entrance into Your glory, that it may soon begin to praise You and bless You forever. Amen. Our Father, Hail Mary, Eternal rest, etc. TUESDAY O Lord God omnipotent, I beseech You by the Precious Blood of Your divine Son Jesus that was shed in His bitter crowning with thorns, deliver the souls in purgatory, and among them all, particularly that soul which is in the greatest need of our prayers, in order that it may not long be delayed in praising You in Your glory and blessing You forever. Amen. Our Father, Hail Mary, Eternal rest, etc. WEDNESDAY O Lord God omnipotent, I beseech You by the Precious Blood of Your divine Son Jesus that was shed in the streets of Jerusalem while He carried on His sacred shoulders the heavy burden of the Cross, deliver the souls in purgatory and especially that one which is richest in merits in Your sight, so that, having soon attained the high place in glory to which it is destined, it may praise You triumphantly and bless You forever. Amen. Our Father, Hail Mary, Eternal rest, etc. THURSDAY O Lord God omnipotent, I beseech You by the Precious Body and Blood of Your divine Son Jesus, which He Himself on the night before His Passion gave as meat and drink to His beloved Apostles and bequeathed to His Holy Church to be the perpetual Sacrifice and life-giving nourishment of His faithful people, deliver the souls in purgatory, but most of all, that soul which was most devoted to this Mystery of infinite love, in order that it may praise You therefore, together with Your divine Son and the Holy Spirit in Your glory forever. Amen. Our Father, Hail Mary, Eternal rest, etc. FRIDAY O Lord God omnipotent, I beseech You by the Precious Blood which Jesus Your divine Son did shed this day upon the tree of the Cross, especially from His sacred Hands and Feet, deliver the souls in purgatory, and particularly that soul for whom I am most bound to pray, in order that I may not be the cause which hinders You from admitting it quickly to the possession of Your glory where it may praise You and bless You for evermore. Amen. Our Father, Hail Mary, Eternal rest, etc. SATURDAY O Lord God omnipotent, I beseech You by the Precious Blood which gushed forth from the sacred Side of Your divine Son Jesus in the presence and to the great sorrow of His most holy Mother, deliver the souls in purgatory and among them all especially that soul which has been most devout to this noble Lady, that it may come quickly into Your glory, there to praise You in her, and her in You through all the ages. Amen. Our Father, Hail Mary, Eternal rest, etc.

Pushing the Limits :: Personal Narrative Papers

Pushing the Limits Late one Friday night in the fall of 1995, while I was hanging out with the girls, my laughter stopped short as Jenny Williams’ large grandfather clock struck one time loudly throughout the old farmhouse. Our laughter and fun came to a halt. My parents’ curfew as well as my school driver’s permit curfew had long expired. Curfew would not have been an issue that night because most fifteen-year-olds would have been at home in bed. Because of my right to drive, my pride in being one of the few freshmen with their school permit came with its own benefits as well as consequences. The privilege to drive to school and back was a privilege easily taken advantage of. One of my rule breaking attempts was driving home from Jenny’s house at 1:00 in the morning. The plan to get home quickly almost backfired and I was almost stuck in a sticky situation, literally! I raced around Jenny’s cluttered house looking for my large wood carved key chain. I knew I needed to make fast tracks before the clock hands worked their way any farther. I found my keys at last and I was racing to my car. My competition with the undefeated clock was soon to begin. I started the engine and took off with the radio blaring like a foghorn, and the accelerator floored. I was about 15 minutes northwest of my house, but I was attempting to cut those minutes in half. As I approached Wellman, I pictured the late shift policeman, Jack Wright, sitting in his car at the town park parking lot just waiting to pull over and ticket some immature teenager like me. I decided to take a short cut down a dirt road, which would allow me to cut some time and avoid any unfriendly policemen. I charged away in my lightweight, manual, gray Chevy Spectrum that I liked to call â€Å"Junior.† While speeding along to Tom Petty’s, â€Å"You Wreck Me Baby,† I had not noticed the shower of mud flying from my cardboard-looking hub capped wheels. The thought crossed my mind to turn back, but the tires had already begun to spin hopelessly like a hamster’s wheel. I realized that turning back towards a possible cop was not an option. This road was not about to let me maneuver a three-point-turn-about either.

Abstract: Understanding the Impact of Childhood Sexual Abuse on Women’s Sexuality

Colangelo, J. J. , & Keefe-Cooperman, K. (2012). Understanding the Impact of Childhood Sexual Abuse on Women's Sexuality. Journal Of Mental Health Counseling, 34(1), 14-37. The purpose of this study was to show how childhood sexual abuse (CSA) victims often are faced with negative psychological and emotional consequences which have profound effects on their lives, as well as their sexuality. The intent of the authors was to show the occurrence of CSA among different women populations, and how it negatively affects them for the rest of their lives.The authors also discuss different suggestions for counselors dealing with these CSA victims, and how they must have a vast understanding of how these women compromise their sex lives because of the trauma. The authors state at the beginning of the article that â€Å"the relationship between child sexual abuse and adult sexual functioning is well-established. †(pg 14) So, the author’s intent with this article was to not only pr ovide additional information regarding CSA victims and how that negatively impacts their lives, but provide insight for counselors who are treating these women.The authors explain the different approaches that counselors must practice, and how these can help the CSA victims. The authors’ intentions were not to do a study, but to help people better understand the issue. Although they pulled different statistics from various articles and books explaining how CSA victims are negatively affected through their sexuality, they provided the readers with a specific example, a case study. The case study addresses the issue by giving an example of a girl named Marie.She was sexually abused as a young girl, but successfully received treatment and counseling, which helped her deal with her CSA issues. After a few years, she found her significant other. The problem was, though, she started experiencing â€Å"intense fear of intimacy, and distressing memories re-emerged. † (pg. 24) The authors go onto explain that the initial counseling did not successfully address the issue with intimacy because she was so young. So, they started a different approach which had three different phases for Marie and her fiance.Phase one was â€Å"Therapeutic Alliance† which focused on establishing rapport between the counselor and Marie, which involved different questions, stories, and advice. This would be the foundation for the entire process. Phase two was â€Å"Addressing CSA Trauma Symptoms† which dealt with all the side effects that Marie was experiencing. She initially had a lot of emotional distress and many different situations triggered the experience where she was sexually abused. The phase-oriented trauma-based approach emphasizes addressing the original sexual trauma as the genesis of maladaptive behavioral responses by working through it, helping women first to relive and revise the traumatic experience and then to acquire healthier coping strategies to replace those created to survive the traumatic experience. † (pg. 26) Phase three was â€Å"Individual Growth and Sexual Development† which centered around addressing different sexual concerns with the couple, but moving at their own pace.As said in the case study, Marie started bettering herself by focusing on her development as a person. Although she was always apprehensive when it came to sexual activity, her issues got better, and this helped the relationship with Bob. Treatment for the couple continued after they got married, which helped them in the long-run. There is a lot of documentation and support providing the huge impact that childhood sexual abuse has on each victim. The aim of this article was to give counselors insight on different treatments, and especially address the specific sexual problems that derive from CSA.The authors discuss treatment techniques and different exercises which help various types of clients that seek treatment. The authors go o nto explain that the counselors are the most vital part of the process, they create an environment where they can better themselves and move past the CSA. The authors of this article want the readers to understand the underlying issues and problems that many women face after they have been sexually abused as a child. Usually people recognize that women face sexual problems after they have been abused, but don’t really understand what they go through, or how to help.So, this can be used as a guide for counselors on CSA victims about who they are, how they react to their situations, and ways they can be successful in the future. By writing this article, the authors wanted to help mental health counselors approach the CSA victims in way where they could be better understood. I found this article persuasive in a sense that the authors make the readers really see what these women go through after they are sexually abused as a child. The authors used plenty of statistics, different references, and thorough information on the sexual disorders that derive from CSA and post-health for the victims.I believe the authors did an agreeable amount of research which persuaded the audience to feel for the victims and really a ppreciate the work that must be done so they can deal with their issues. One of the only issues I had was that the authors did not conduct an experiment of their own. I was hoping to see a survey that they had researched and developed, but their case study was more of the focal point of the article. I believe that if they would have done an actual research method themselves, rather than using statistics from elsewhere, there would have been a little more support for their argument.In my opinion, the article was very easy to understand and well written. Each section had its own title, different paragraphs, and organized accordingly. I like the fact that the researchers had a specific case study in which they presented in the article. I believe it he lped the readers understand the article better, and seeing a true example validated the information. By organizing it into different sections, it helped me understand the content better and made reading it easy. Also, this helped me want to read more.Once I read the introduction, I kept wanting to read further, and learn more. The authors did a good job at making me think about the subject matter and get me excited to learn about it. Lastly, the authors of the article did a decent job at tying in other literature and different research. Like I said earlier, they mainly pulled statistics and other information from books and journals, and this supported their claims. By using a third party for more information, this helped the audience understand the problems better.

Commercial Accounting Essay

Commercial accounting is an accurate account of business transactions, it can only be acquired if both cash and credit transactions are performed, recorded, and recognized in such a way that there can be no duplication and the transactions are recorded under commercial accounting. The system known as double entry book keeping or the commercial accounting system that records both aspects of transactions, also it records credit transactions in cash accounting the day of the receipt. The single entry in accounting for example: there is a sale, where the transaction is complete after the exchange of cash for goods. In commercial accounting the date in which the transaction was made needs to be recorded regardless to whether it was paid or it wasn’t. Generally Accepted Accounting Principles better known as (GAAP) is the collection of standards and practices that are required to be used by business’s to record and present the results of their financial activities and their re cords of what they owe, established by the Financial Accounting Standards Board (FASB). These accounting principles are standardized and used for the many private and publicly traded companies in America today. Every economic event within business must be associated with and recorded by a specific entity and business records must not include the personal assets or liabilities of the owner. If an idea holds no impact on a decision maker, investors, or creditors, then the relating (GAAP) principles do not have to be followed. (GAAP) requires the use of accrual basis accounting rather than cash basis accounting, this is imposed on businesses so that investors have a minimum level of consistency in the financial statements they use when analyzing businesses for investment purposes. The accrual basis of accounting over the cash basis of accounting in commercial accounting and generally accepted accounting principles (GAAP) is different depending on whether you run a large scale business or a small business. Cash basis is used mostly by small businesses where owners and creditors want a simple way to understand the financial statements and to understand whether there is a profit or loss in the business. Accrual basis is used by large businesses and when revenue is earned expenses are accrued. Cash basis gives you a clear picture of your cash flow, but does not give you a good idea of business in the long term because you are not recording income when it is actually earned. Accrual basis accounting accurately matches your income and expenses and if not managed carefully you could lose track of your actual cash flow, with accrual basis transaction statements they are recorded in which the events occur. You don’t have to wait until you see the money, or actually pay money out of your checking account, to record a transaction. References (Investopedia.com) (Wiley PLUS)

Canine Parvovirus

Canine Parvovirus Free Online Research Papers It is extremely important to make sure all puppies get proper immunizations for the Canine Parvovirus or Erythema infectionism. There is no specific treatment or cure for this virus. There is only supportive care. The virus can have deadly side effects and be extremely hard to destroy (Staff, 2008). â€Å"The Parvovirus is able to withstand a wide range of PH levels† (the Merck Veterinary Manual 9th Edition, 2005, p316). The Canine Parvovirus is very hard to destroy and can have very devastating effects. â€Å"The origin of the Parvovirus is unknown† (The Merck Veterinary Manual 9th Edition, 2005, p317). In the beginning there were two types of the Canine Parvovirus Myocarditis and Gastroenteritis. Myocarditis occurred in puppies still in the neonatal period. This type is almost never seen anymore because of immunizations. Gastroenteritis is the common type of Canine Parvovirus and usually effects dogs six to twenty weeks old. The Merck Veterinary Manual 9th Edition says, â€Å"Males are more likely to develop Gastroenteritis than females because of their tendency to roam† (p.317). In the 1980’s type CPV (Canine Parvovirus)-2a became popular, and then in 1986 CPV-2b replaced CPV-2a. Recently they have discovered new strands of the Parvovirus that are more difficult to test for. â€Å"Today CPV-2b has largely replaced the previous strands† (Doctors Foster and Smith, 1997-2009,  ¶ 2). The most recent strand discovered is CPV-2c, and they think there are more yet to be named. These new strands are more difficult to test for and are more resistant to vaccinations (Doctors Foster and Smith, 1997-2009). â€Å"The only effective disinfectants are bleach and products labeled specifically for killing the Parvovirus† (staff, 2008,  ¶11). It survives indoors for only about a month, but outdoors it can stay active for months depending on the conditions. According to the Mayo Clinic Staff, â€Å"the Parvovirus should be considered contagious in shady areas for seven months and areas with sunlight for five months, but if it is freezing the Parvovir us will not die† (Staff, 2008,  ¶ 3). Recovered dogs can spread the virus by being carriers. The virus can also be spread by feces of infected dogs. This â€Å"shedding occurs four days after infection and peaks when clinical signs appear† (The Merck Veterinary Manual 9th Edition, 2005, p317). The Merck Veterinary Manual 9th Edition says, â€Å"Rottweiler’s, American Pit Bull Terriers, Doberman Pinchers, and German Shepherds are at increased risk, while Toy Poodles and Cocker Spaniels seem to be at decreased risk† (The Merck Veterinary Manual 9th Edition, 2009, p316). Parvovirus can survive everywhere so it is considered â€Å"ubiquitous† (Staff, 2008,  ¶ 4). A dog usually gets the virus inside of the body through the mouth. The virus lives on all surfaces so when a dog licks themselves, or any object, they are exposed. Dogs also get the virus when they eat feces (Staff, 2008). When the Parvovirus enters the body it goes to the Lymph System and stays there multiplying for about three to four days. Then the virus begins to spread to the organs where it can multiply faster. From there it travels to the bone marrow. When the Parvovirus is in the bone marrow it attacks the immune system. This makes the body almost defenseless as the virus moves to the stomach (Staff, 2008). Once the Parvovirus is in the GI tract it begins to destroy the intestinal cells. It attacks the â€Å"Crypts of Lieberkuhn†, (Staff, 2005,  ¶ 10) which is where new cells for the intestines are created. When new cells cannot be created the villus begins to die. This causes major problems because the villus absorbs nutrients from food and water. This also destroys the lining of the stomach causing more problems. Diarrhea and nausea are major side effects of this destruction. Dehydration and the body becoming sepsis are the most common causes of death with the Parvovirus. The body can become septic when the lining of the stomach is destroyed and bacteria can enter the bloodstream (Staff, 2008). No test is completely foolproof so it is recommended that more than one test be used to test for the virus. When the Parvovirus attacks the immune system it destroys the white blood cells, this can be seen in blood tests. The most effective and most widely used test is the ELISA test. â€Å"The ELISA is short for Enzyme Linked Immuno Sorbant Assay. It is similar to a pregnancy test but uses a stool sample† (Staff, Diagnosis, 2005,  ¶ 2). There are different brands but they all are effective and take about fifteen minutes for the results; however, if a puppy has had a live vaccine in the past five to twelve days it can affect the test results (Staff, 2008). Veterinarians used to run an IgG or IgM titer to test for the virus. This is not common anymore because the IgG titer will be positive if the puppy has had a live vaccine recently. The IgM titer only shows true results if there is increased activity in puppies that have not been recently vaccinated. These tests are difficult to read and are very limited. (Staff, 2008) The most common form of treatment is giving fluids to help prevent dehydration. â€Å"Dogs are given oral electrolytes if dehydration is mild and there is no vomiting, but if dehydration is severe and vomiting exists electrolytes are given intravenously† (The Merck Veterinary Manual, 2005, p 320). It is only recommended to give antibiotics if â€Å"there is blood loss, fever, or loss of intestinal integrity† (p320). When antibiotics are used they are given in combinations to be more effective. The Merck Veterinary Manual 9th Edition says, â€Å"the most common antibiotics are Ampicillin or first or second generation Cephalosporin and Aminoglycosodine or Enrofloxacin (p320). Because there is such little treatment, the risk of exposure is great, and effects of the virus are so severe vaccinations are crucial (The Merck Veterinary Manual 9th Edition, 2005). References Doctors Foster and Smith. (1997-2009). Retrieved February 12, 2009, from Live Aquaria.com: peteducation.com/article.cfm?c=1556aid=467 (2005). The Merck Veterinary Manual 9th Edition. In The Merck Veterinary Manual 9th Edition (pp.319-324). Whitehouse Station, N.J.: Merck and Co., INC. Staff, M.C. (2008, November 15). Canine Parvovirus. Retrieved January 21, 2009, from http://mayoclinic.com/healthhdl-choleserol/CL00030:mayoclinic.com Research Papers on Canine ParvovirusSexually Transmitted DiseasesMRSA Staph BacteriaImpact of Breast CancerYear Round SchoolingGenetic EngineeringIs the Use of Psychotropic Drugs in the Treatment ofLove DivorceThe Legal ProcessCash or Card?Project Management 101

Flores Surname Meaning and Origin - About Genealogy

Flores Surname Meaning and Origin - About Genealogy The surname Flores has existed in Spain since the 12th century, but a common origin has not been found. It is believed to most likely derive from the given name Floro, meaning flower from the Latin flos. Flores is the 55th most popular surname in the United States and the 15th most common Hispanic surname. Surname Origin:  Spanish Alternate Surname Spellings:  FLOREZ, FROYLEZ, FROLAZ, FLORIZ, FLORAZ, FLOR, FLORAN, FLORIAN, FLORIO, FLORIS, FLORI Famous People With the Surname FLORES Pedro Flores - inventor of the yo-yoFrancisco  Flores  Perez - former president of El SalvadorJuan Josà © Flores  - first president of EquadorMarco Antonio Flores  - Guatemalan writer and poetJosà © Asuncià ³n Flores  - Paraguayan composerSalvador Flores -  Alamo defender; recruiter of volunteers in support of the Texas Revolution Where Do People With the FLORES Surname Live? The surname distribution data at  Forebears  ranks Flores as the 167th  most common surname in the world, identifying it as most prevalent in Mexico and with the highest density in Honduras. The Flores surname is actually the most common surname in the nation of Bolivia, and also ranks among the top ten last names in Peru (2nd), El Salvador (4th), Honduras (5th), and Guam (10th) and Mexico (10th). It is also common within Guatemala, Chile, Venezuela, Belize and Argentina.  Within Europe, Flores is most frequently found in Spain, according to  WorldNames PublicProfiler, especially the western provinces of  Badajoz and  Cceres. The Flores surname is also found throughout the United States, where it is most common in the states of California and New Mexico. Genealogy Resources for the Surname FLORES 100 Most Common Spanish SurnamesHave you ever wondered about your Spanish last name  Ã‚  and how it came to be? This article describes common Spanish naming patterns, and explores the meaning and origins of 100 common Spanish surnames. How to Research Hispanic HeritageLearn how to get started researching  your Hispanic ancestors, including the basics of family tree research and country specific organizations, genealogical records, and resources for Spain, Latin America, Mexico, Brazil, the Caribbean and other Spanish speaking countries. Flores Family Crest - Its Not What You ThinkContrary to what you may hear, there is no such thing as a Flores family crest or coat of arms for the Flores surname.  Coats of arms are granted to individuals, not families, and may rightfully be used only by the uninterrupted male line descendants of the person to whom the coat of arms was originally granted.   Flores Family Genealogy ForumSearch this popular genealogy forum for the Flores surname to find others who might be researching your ancestors, or post your own Flores query. FamilySearch - FLORES GenealogyAccess over 6.3 million free historical records and lineage-linked family trees posted for the Flores surname and its variations on this free genealogy website hosted by the Church of Jesus Christ of Latter-day Saints. FLORES Surname Family Mailing ListsThis free mailing list for researchers of the Flores surname and its variations includes subscription details and a searchable archives of past messages. DistantCousin.com - FLORES Genealogy Family HistoryExplore free databases and genealogy links for the last name FLORES. The Flores Genealogy and Family Tree PageBrowse family trees and links to genealogical and historical records for individuals with the last name Flores from the website of Genealogy Today. - References: Surname Meanings Origins Cottle, Basil.  Penguin Dictionary of Surnames. Baltimore, MD: Penguin Books, 1967. Dorward, David.  Scottish Surnames. Collins Celtic (Pocket edition), 1998. Fucilla, Joseph.  Our Italian Surnames. Genealogical Publishing Company, 2003. Hanks, Patrick and Flavia Hodges.  A Dictionary of Surnames. Oxford University Press, 1989. Hanks, Patrick.  Dictionary of American Family Names. Oxford University Press, 2003. Reaney, P.H.  A Dictionary of English Surnames. Oxford University Press, 1997. Smith, Elsdon C.  American Surnames. Genealogical Publishing Company, 1997.

A Small Scale Study on the Perspectives of Saudi Undergraduate Assignment

A Small Scale Study on the Perspectives of Saudi Undergraduate Students on the Use of Smartphones as a Motivational Learning Tool in Preparatory Year Language - Assignment Example he primary language of international trade, science and technology and since Saudi Arabia is the world’s largest oil producing country, English is perceived as an integral part of the human resource development agenda of the Saudi government (Alseweed, 2009). Given the Saudi government’s commitment to improving English language skills in Saudi Arabia as evidenced by 6 years of compulsory English at school and the unsatisfactory results, several studies have been conducted on identifying the reasons for the unsatisfactory acquisition of English language among Saudi students and how to best motivate Saudi students for improved results (Alsewee, 2009; Liton, 2012; Khan, 2011). Informed by adult learning theories, this research proposes to investigate the effectiveness and efficiency of smartphone use in preparatory year language learners in Saudi Arabia. Adult learning theories inform that adult learners are more complex than children learners and that as adults, experienc es together with fixed opinions and perceptions instruct that adults are more amenable to learning that takes account of their specific needs and interests (Hough, 1984). Barrs (2011) considered the learning needs and interests of young adult students and the advances in mobile technology and argued that smartphones in particular have a particular value as a language learning tool for young university students. For the most part, these students own and use smartphones regularly. Moreover, young university students also rely on a number of smartphone applications for sharing and processing information and have access to this information via smartphones on a continuous level. Therefore smartphones can be used to channel the interest, experience and technological needs of young adult students as a... This study will therefore contribute to adult learning theories and the exploration of the possibility of smartphone technologies as a means of enhancing Saudi Arabia preparatory year student’s acquisition of a second language. A qualitative case study will be conducted among male students in the preparatory year at a college in Saudi Arabia. A phenomenological approach will be taken in that students will be interviewed as a means of discovering their experiences with leaning English and their attitudes toward smartphones as a learning tool and how this correlates with their interest and learning needs. The aim of this proposed research is to determine the extent to which the use of smartphones is a viable learning tool for improving the language proficiency of preparatory year language students in Saudi Arabia. Since this research focuses on a specific programme in Saudi Arabia, it is a social phenomenon that is more appropriately investigated using a qualitative case study. Since this research is investigating an educational programme with a view to determining whether or not the use of smartphones can improve language proficiency and thus motivational learning, an illustrative case study will be conducive to achieving this research objective. The illustrative case study will allow for the collection of rich and detailed person-centred data, describing individual perspectives and feelings and thus informing whether or not smartphones are appropriate learning tools for young adult language learners in Saudi Arabia’s preparatory language programme.

Published opinion of Rosa Brooks from Los Angeles Times analysis Personal Statement

Published opinion of Rosa Brooks from Los Angeles Times analysis - Personal Statement Example Because as economists put it, when US cough, the world catches cold. The recent years have seen the stronger and tighter relations among nations as they open up their borders for freer trade through the formation of trade blocs and regional partnership. Aside from local produce, most of the consumers' income in US is spent in cheaper imported goods coming from Asian trading partners like China, Philippines, Malaysia, and Thailand. These nations have long been the biggest trading partners of US and have long benefited from the strong demand in our country. With the technological development, services have also been imported from these nations as well through business process outsourcing. So, with these nations heavily dependent on US partnership, it is irrefutable that the economic recession in our country will also bring about slowdown in other smaller and less stable economies. On the other hand, the collapse of Lehman Brothers is also expected to sabotage growth in the Eurozone. So, it's not just the US which should be given with this letter and th e much needed economic assistance from IMF and WB.

REFLECTION ON TRANSITION ACTIVITIES Essay Example | Topics and Well Written Essays - 2750 words

REFLECTION ON TRANSITION ACTIVITIES - Essay Example The program aims to improve lifestyle of diabetic patients and learn the various perspectives on the management of diabetes more effectively. In this regard, the essay aims to proffer my personal reflection on the specific transition activity focusing on diabetic management. In the process, an examination of the learning modules would provide ample information on areas where evidence contradicts reality. As such, the discourse would reveal how the transition activities have contributed towards the honing of personal and professional skills. An assessment of these skills through the transition activity would enable me to gauge specific areas which need to be improved and enhanced. Finally, the reflection would reveal specific scenarios to support the contentions in the essay. The diabetes management program at Lakeside Medical Center clearly outlined the following learning objectives, to wit: classification of Diabetes Mellitus, Insulin Therapy, Management of Newly Diagnosed, and the Science of Nutrition, among others. I attended four (4) sessions specifically detailed as follows: The first session was learning about â€Å"What is Diabetes (exploring diabetes, lifestyle, etc.). The second session focused on carbohydrates (CARB) awareness including the amount and types of carbohydrates. The third session detailed reading and understanding food labels. Finally, the fourth session discussed possible short term and long term complications of diabetes and understanding medication and health profile. From the modules, I learned that nursing care for diabetes encompasses a combination of glucose monitoring, specialized diet, regular exercise and proper medications. Since diabetes is considered a chronic illness, patients diagnosed with the disease must be made aware that the responsibility and accountability of daily management of one’s illness is the patient himself. The

Nuclei And Mitochondrial Fraction

Nuclei And Mitochondrial Fraction The objective of this experiment is to prepare a nuclei and mitochondrial fraction using differential centrifugation, from a rat liver homogenate sample. The amount of activity of mitochondria in the fractions can be measured using succinate dehydrogenase (SDH) as a marker. To measure the percentage recovery of the SDH of Mitochondrial, Nuclei and supernatant fractions in comparison to the Homogenate and to Calculate the specific and relative activity of SDH in each fraction. Figure 1: Shows a typical animal cell with the individual organelle components. Figure 2: Shows the typical features and functions of the organelles of interest in this report. Figure 1 + 2 Created on Microsoft paint with reference to Essential Biology (2004) Individual organelles differ in size but are all usually around 10nm in diameter. There is a small surface area and size/density depends on the organelle, the smaller organelles being lysosomes and ribosomes. Mitochondria differs in cell type depending on the energy demand of that organ, the more ATP that is required in a particular organ the more mitochondria found. E.g. more mitochondria found in heart and liver cells than in a white blood cell like a lymphocyte. Smaller organelles include lysosomes and ribosomes. Metabolism can be detected using various methods such as use of inhibitors. These can be both competitive and non-competitive, an example is seen with arsenic with inhibits pyruvate dehydrogenase. Another method is with the use of radioisotopes to measure activity aswell as histochemistry, immunocytochemistry and electromicroscopy. Preparation of the homogenate occurs in various stages. Firstly the homogenisation of liver cells. This can be done using a Potter Elvehjem homogeniser to extract organelles without damaging the actual cell. This is a simple and effective homogenisation method. A small gap is made within the cell wall which is then pressurised which forces the contents i.e organelles, cytoplasm etc. out of the cell. This occurs at a low temperature and mild pH, and to keep the isotonic solution a sucrose buffer is used, therefore since there is the same water potential inside the cell and outside the cell there is no net movement of water (osmosis) and thus the cell remains the same size. Homogenized cells also must be kept at low temperatures to prevent autolysis (the degradation of a cell by its enzymes). (www.bookrags.com). Figure 3 shows a classic Potter Elvehjem homogeniser Image taken from (umwcellbiology.org) The second stage is fractionation of the homogenate sample. This process is called centrifugation and can be further split into either a differential centrifugation or a density gradient centrifugation. The differential centrifugation splits the impure fraction into separate compartments due to the size of the various organelles in question and there density. The centrifuge applies a gravitational force onto the sample to separate components. The rate of centrifugation is determined by the acceleration or speed applied to the homogenate and is usually measured in revolutions per minute (RPM) or g. Depending on the density of the organelles will determine their isolation at a given speed. The higher density organelles and the bigger organelles separate at a lower speed centrifugation. (K. Wilson 2005). The separation forms a pellet which is the precipitate proportion of the sample and the component of interest and a supernatant which is the liquid component. The supernatant readily de canted from the sample without removing the precipitate. Diferemces in centrifugation occur due to the techniques used, differential centrifugation is based upon the sedimentation rate of particles and thus the sedimentation rate separates them based on size and density. After initial sedimentation the largest particles separate first into pellet and supernatant (K. Wilson 2005). Density gradient centrifugation separates organelles using a media. Various media can be applied and depending on the particles will be best for certain types and may not work well for others. (K. Wilson 2005). The 4 fractions we will obtain are nuclei, mitochondrial, supernatant and homogenate. Various tests can be carried out to distinguish between fractions and to determine their actual purity, testing for specific enzymes can code for the activity occurring in the cell fractions therefore indicating the most abundant component. Some tests include: Testing for DNA in both nuclei and mitochondrial fractions. This is because DNA is contained within the nucleus but also within the mitochondria. This is because relating to the endosymbiotic theory mitochondria was a separate aerobically respiring bacterial cell which was later engulfed by an early eukaryotic cell to merge into one aerobically respiring cell. Mitochondria is maternally inherited in the case of the majority of multicellular organisms, this is due to the higher number of mtDNA molecules in the ooecyte and much fewer in a sperm cell which are mostly degraded before fertilization takes place. Test for histones which indicate nuclei fraction as well as testing for various enzymes such as ATPase found in cytoplasmic (supernatant) and mitochondrial fractions and phosphotase kinase indicating microsomes and golgi apparatus are present. Some enzymes are exclusive to the citric acid cycle which occurs in the mitochondria, therefore testing for these enzymes indicates the presence of mitochondria in a fraction. The enzyme marker to test for mitochondria which we use is succinate dehydrogenase which is exclusive to the inner mitochondrial membrane. Succinate dehydrogenase is formed only during the citric acid cycle so is only given as an indication of mitochondria. However, since during the homogenisation process the mitochondria could potentially burst spilling their contents into the cytoplasm (supernatant fraction), this does not therefore give an accurate indication of mitochondria present in a fraction. Succinate dehydrogenase breaks down succinate into fumurate, therefore t he measurement of formazan indicates presence of succinate dehydrogenase. Measuring Succinate Dehydrogenase Activity (Red Formazan assay) This occurs in 2 reactions: 1: succinate + FAD à ¨ fumarate + FADH2 SDH breaks down succinate into fumarate. This is an oxidation reaction since the succinate loses 2 electrons, in addition a reduction of the enzyme flavin adenine dinucleotide occurs (FAD gains 2 electrons) (FAD + 2 electrons à ¨ FADH2) Figure 4: Shows the redox reaction which occurs with succinate and FAD. Image taken from natuurlijkerwijs.com SDH activity is measured by the formation of formazan a deep red compound formed from the reduction on a tetrazolium salt. The reduced FADH2 reduces tetrazolium salt (INT). 2: FADH2 + INT à ¨ FAD + formazan Centrifugation and calculating the relative centrifugal field. (K. Wilson 2005) G = W2r = 4 II2 r (rPM)2 = 1.1110-5r (rPM)2 3600 G= Relative centrifugal force (RFC) r = Radical distance from axis of rotation w = Angular velocity rPM = Revolutions per minute. T = 9 É ² (In Rt/Rb) 2 W2rp2 (Pp -P) É ² = Viscosity of medium rp = Radius of particle Pp = Density of particle P = Density of medium Rt = Radius to top of centrifuge tube Rb = Radius to bottom of centrifuge tube. There are many differences in types of centrifuges available and results depend on the speed of the centrifugation and whether a vacuum is present and the type of rotor used. (K. Wilson 2005) Analysis of marker enzymes in subfractions determines the recovery of subcellular organelles, with comparison to previous tests, quantative data can be used to assess contamination of fractions. Showing whether the subfractionation method has been successful or not. These tests also hold health benefits and implications e.g. microsome C causes cell death and can be found in mitochondrial fractions, however in cancer patients no microsome c is present, indicating no cell death will occur a common feature of cancer cells. Enzyme measurement in subcellular fractions however does hold some implications such as the solubility of the environment which may cause differences in enzyme function. Another implication is latency of enzymes, this refers to whether proteins are bound to the enzyme which in turn activates them once bound signalling enzyme function. There may also be low recovery of enzymes in the fractions due to poor recovery of the organelles which they come from, in particular if the enzyme is confined only to a specific region. Over the 3 week period centrifugation will separate the fractions according to size/density and separating the sample into the pellet and supernatant fractions. The speed of the centrifuge determines whether the pellets will separate. A lower speed is needed to separate the nuclei fraction due to the higher density, whereas the higher speed is needed to separate the supernatant due to the smaller density remaining organelles. (K. Wilson 2005). The protein content is also measure for each fraction using the biuret assay, absorbance values are given which determine the protein content of each fraction. Finally succinate dehydrogenase is measured. This causes a redox reaction and causes e- ions are released, using formazan as an indicator this changes the colour of solution red, showing a redox reaction has taken place. From this research I can predict that the mitochondrial fraction is expected to have the highest results in specific activity due to fewer proteins present in that fraction. Results: Calculations: Formazans molar extinction coefficient (E490nm) = 20,100 M-1 cm-1 The specific activity and relative activity of the fractions can be determined by measuring the concentration using Beer- Lamberts Law: (www.chemguide.co.uk) A = ÃŽ µ x l x C A = Absorbance (no units) ÃŽ µ = Epsilon. The adsorbtion coefficient M -1 cm -1 l= Cuvettes light path length, this is the length of solution a light passes through (always 1 cm) C= Concentration of substance in M (moles in 1 litre) Rearrange to give concentration: C = A / ÃŽ µ x l Units: M-1 x cm-1 = 1 / M x cm C = A / ÃŽ µ x l Gives units: ( 1/ (1/M x cm) x cm). This can be simplified to give 1/ (1/M) And further simplified to give units: M (moles per litre or dcm -3) Know values: ÃŽ µ = the formazan adsorption coefficient is 20,100 M -1 cm -1 A = refers to the absorbance at 490nm values for each fraction are found in the mean-control table section. Using the equation: C = A / ÃŽ µ x l We can work out the concentration of formazan formed in the reaction. The concentration value is for 1 litre, therefore we must calculate the actual concentration from the actual assay volume used. Concentration = amount/volume rearranged to give A = C x V The final assay volume from week 3 is 6 ml* due to the addition of ethyl acetate. * Note by mistake 6ml of ethyl acetate was added instead of 4 ml giving a different final volume to the other groups. Converting 6ml into its litre value and x by the concentration gives the accurate mole product of formazan produced. Reaction time needs to be included to give the accurate units. Activity units can be determined using the following equation. Activity = Moles of formazan/reaction time (12 minutes) This gives the activity in M -1 Calculating total activity and specific activity of the fractions. Table 1: Total volumes from each cellular fraction. Fraction Total Volume (ml) Homogenate 12 Nuclei Fraction 12 Mitochondrial Fraction 12 Supernatant Fraction 26 To do this we need to take into the account: The total volume The total protein of the fraction. Dilution factor The total volume values for each fraction can be found in table 1. The sample of each fraction used was 0.2ml, therefore the amount of moles of formazan calculated is in 0.2ml. (0.2 / total volume) x moles of formazan in 0.2ml X by the dilution factor of each fraction to give the total activity for each fraction, the values are given in table 4. To determine the specific activity we must consider the total protein of the fraction. Values are given in table 3. Specific activity = Total activity of fraction/ total protein of fraction Table 2: Bovine serum albumin (BSA) solution concentrations Volume (ml) BSA (10mg/ml BSA 0 0.2 0.4 0.6 0.8 1.2 1.6 0.1m NaOH 2.0 (blank) 1.8. 1.6 1.4 1.2 0.8 0.4 Table 3: Values for BSA standard curve. See Graph 1 for the results from the corresponding fraction absorbance. Protein Amount (mg) 0 (blank) 2 4 6 8 12 16 Absorbance at 550nm 0 0.105 0.184 0.275 0.354 0.511 0.531 Table 4: Protein amount in homogenate and subcellular fractions. Homogenate 0.05ml Nuclei 0.2ml Mitochondria 0.2ml Supernatant 0.2ml Average Absorbance (550nm) 0.169 0.054 0.174 0.199 Protein amount in samples aliquot (mg) 3.6 1.18 3.8 4.15 Protein concentration in fraction (mg/ml) 72 5.9 19 20.75 Protein amount in fractions total volume (mg) 864 70.8 228 539.5 Graph 2: Shows the difference in protein amount amongst cellular fractions. Table 5: Actual concentration of fraction after dilution. Dilution Factor Actual concentration (mg/ml) Homogenate 20 3.6 Nuclei 3 2 Mitochondrial 20 0.95 Supernatant 1 20.75 Table 6: Formazan content absorbance at 490nm. Fraction Control Test 1 Test 2 Mean-Control Homogenate 0.132 0.58 0.52 0.42 Nuclei 0.21 0.352 0.326 0.13 Mitochondrial 0.057 0.391 0.265 0.27 Supernatant 0.132 0.52 0.33 0.29 Results for Homogenate: From table 5, we have the absorbance of homogenate as 0.42 this divided by the adsorption co-efficient gives: 0.42/20,100 = 2.1 x 10 -5 M The units for concentration are left as moles per litre (M). To get this into moles in the actual volume used (6ml not 1 litre) 2.1 x 10 -5 M x 0.006 lite = 1.3 x 10-7 M Include the reaction time of 12 minutes to give moles per minute. 1.3 x 10-7 M /12mins = 1.010-8 M -1 To determine total activity and specific activity. The total volume from table 1: for the homogenate is 12ml, however the sample used was only 0.2ml we therefore divide actual volume / used volume x concentration of H x dilution factor (20 in the case of the homogenate from table 5 values) Total activity = (12/0.2) x1.0x10-8 M -1 x 20 = 1.2 x 10 -5 M -1 specific activity = 1.2 x 10 -5 M -1/ total amount protein in homogenate from table 4 1.2 x 10 -5 M -1/864= 1.3 x 10-8 M min-1 Results for nuclei fraction: 0.13/20,100 M-1 cm-1 = 6.5 x 10-6 In 0.006 litre : 6.5 x 10-6 x 0.006 = 3.9 x 10-8 M 3.9 x 10-8 M / 12 = 3.2 x 10-9 M min-1 Total activity = 3.2 x 10-9 M min-1 x (12/0.2) x 3 = 5.8 x 10 7M min-1 Specific activity = 5.8 x 10 7/ 70.8 = 8.2 x 10 -9 M min-1 Results for mitochondria: C = 0.27/20,100 m-1 cm -1 = 1.3 x 10-5à ¢Ã¢â€š ¬Ã¢â‚¬ËœM 1.3 x 10-5à ¢Ã¢â€š ¬Ã¢â‚¬ËœM x 0.006 = 7.8 x 10-8 M 7.8 x 10-8 M / 12 = 6.5 x 10-9 M min-1 Total activity = 6.5 x 10-9 M min-1 x (12/0.2) x 20 = 7.8 x 10-6 M min-1 Specific activity = 7.8 x 10-6 M min-1/228 = 3.4 x 10-8 M min -1 Results for supernatant: C = 0.29/20,100 m-1 cm -1 = 1.4 x 10-5à ¢Ã¢â€š ¬Ã¢â‚¬ËœM 1.4 x 10-5à ¢Ã¢â€š ¬Ã¢â‚¬ËœM x 0.006 = 8.7 x 10-8 M = 8.7 x 10-8 M / 12 = = 7.3 x 10-9 M min-1 Total activity = 7.3 x 10-9 M min-1 x (26/0.2) = 9.4 x 10-7 M min-1 Specific activity = 9.4 x 10-7 M min-1/539.5 = 1.7 x 10-9 M min -1 Percentage recovery of Succinate Dehydrogenase for the fractions This is done by dividing the amount of Succinate dehydrogenase in the individual fractions by the original homogenate and then multiplied by 100 to give a percentage. Table 7: Shows the total activity for each of the fractions. Fraction Total Activity Homogenate 1.2 x 10 -5 M -1 Nuclei 5.8 x 10 7M min-1 Mitochondria 7.8 x 10-6 M min-1 Supernatant 9.4 x 10-7 M min-1 Nuclei fraction: (5.8X10-7/1.210-5 ) x 100 = 4.8% Mitochondria fraction (7.810-6/1.210-5 ) x 100 = 65% Supernatant fraction (9.410-7/1.210-5) x 100 =7.8% Relative Specific Activity of Succinate Dehydrogenase This is found by dividing the specific activity of the fractions (found above) by the specific activity of the homogenate (found above). Table 8 shows the specific activity for each of the fractions: Fraction Specific Activity Homogenate 1.4 x 10-8 M min-1 Nuclei 8.2 x 10 -9 M min-1 Mitochondria 3.4 x 10-8 M min -1 Supernatant 1.7 x 10-9 M min -1 Nuclei fraction 8.2 x 10 -9 M min-1 /1.4 x 10-8 M min-1 = 0.586 Mitochondrial fraction 3.4 x 10 -8 M min-1 /1.4 x 10-8 M min-1 = 2.429 Supernatant fraction 1.7 x 10 -9 M min-1 /1.4 x 10-8 M min-1 = 0.121 Discussion: Note: There was very little protein found in the nuclei fractions total volume, this is abnormally low since we would expect this to be higher. From the results we can determine that the this supports our prediction that The mitochondrial fraction is expected to have the highest results in specific activity due to fewer proteins present in that fraction. Organelles have been isolated from each other as seen with the differing proportions of protein found in each fraction as well as the differing values for specific and total activity calculated. However the homogenate is expected to have the highest total activity due to the higher amount of protein since all fractions are present. However since protein was found in the cytoplasm or supernatant fraction, this indicates that there was an error in the separation of the fractions as SDH is present where it usually isnt found. Succinate dehydrogenase works by transferring 2 electrons from succinate which transfers it to fumerate, which blocks the rest of the reaction when it binds to FAD, from the measurement of formazan gives the value of activity. Results show that the relative specific activity is highest in the mitochondrial fraction, as well as the percentage recovery of the fractions. Therefore demonstrating that the fractions were purified and that the homogenisation and centrifugation has been relatively successful in separating fractions. However there were some inaccuracies from the results, this includes the very low protein amount found with in nuclei fraction, this was however predicted to contain a higher amount of protein due to the nature of the organelle and the enzymes contained within it. Another inaccuracy in this experiment is that SDH was found within the supernatant. This is primarily a marker for mitochondria so would not usually be found within the cytoplasm, however due to mitochondria bursting and releasing its contents into the cytoplasm during the homogenisation stage and centrifugation the enzyme succinate dehydrogenase was present. Since the test was carried out under the same conditions in a neutral pH buffer we can conclude that this was a fair test, however is it often found that the more molecules present in a The separating of the homogenate could be improved by using another method of homogenisation, in this experiment we used a Potter Lethem homogeniser which is a glass and plastic hand homogeniser. This perhaps isnt the most accurate at pressurising cells with the force needed to accurately release cell content. Alternative homogenisers include ultrasonic and rotor based homogenisers which may provide more accurate. (www.proscientific.com) A different centrifugation method used. During this experiment differential centrifugation was used, however density gradients may provide more accurate at purifying a sample (www.coleparmer.co.uk). This method works by placing various layers after layer of gradient media such as sucrose in a tube with the heaviest layer at the bottom and the lightest at the top. The cell fraction to be separated is placed on top of the layer and centrifuged. Density gradient separation can be classified into two categories. Rate-tonal (size) separation. Isopycnic (density) in which organelles separate until their density matches the surroundings of the media in which they are. A very good medium for separating organelles is an iodinised media. (www.coleparmer.co.uk). Accuracy of the absorbance and accuracy of obtaining the protein amount. Results are slightly low indicating inaccuracy in both collecting the samples and also measuring the absorbance, this could be due to error in homogenisation and centrifugation techniques but could also be due to error in the reading of absorbance using the Spectrophotometric assay since U.V wavelength has different absorbance levels if either oxidised or reduced enzymes absorb light therefore giving innacurate indication to enzyme present (www.millipore.com) . This may affect the absorbance levels in the fractions if specific enzymes are affected thus giving an altered absorbance level and therefore undermined protein amount. Another method to measure enzyme assay could be to use a caliometric method which measures heat radiance given off instead of the absorbance levels. Some of the organelles which remain in the supernatant fraction are the smaller and less dense proportions of the cell such as ribosomes and lysosomes. Further centrifugation at a higher speed can be used to separate these smaller less dense organelles into pellets. This can also be used to further purify bacteria. In conclusion we see that as predicted, the specific activity is highest in the mitochondrial fraction and the total activity is highest in the homogenate. The % recovery of each fraction and the relative specific activity for each fraction calculated shows a higher proportion in the mitochondrial fraction also. Overall the results indicate accurate laboratory skills and results conclude what was intended, however some slight changes to laboratory equipment would mean that some of the results such as SDH found in the supernatant may not come about in a future test.